Methotrexate

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Methotrexate API Reference standards and its EP Impurities, USP Impurities, non pharmacopeial Impurities, listed below & also offered custom synthesis for unknown Impurities. If require any contact info@drjcrbio.com, sales@drjcrbio.com

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    Product Name : 2,4,5-Triamino-6-hydroxypyrimidine Sulfate/35011-47-3

    Code: JCMTT-21
    CAS: 35011-47-3
    Chemical Formula: C4H7N5O·H2SO4
    Molecular Weight:239.21
    Category: Methotrexate Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: 2,4,5-Triamino-6-hydroxypyrimidine Sulfate/35011-47-3 is useful as reference Impurity standard.
    2,4,5-Triamino-6-hydroxypyrimidine Sulfate/35011-47-3
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    Product Name : (2,4-Diaminopteridine-6,7-diyl)dimethanol

    Code: JCMTT-20
    CAS: NA
    Chemical Formula: C8H10N6O2
    Molecular Weight: 222.20
    Category: Methotrexate Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: (2,4-Diaminopteridine-6,7-diyl)dimethanol is useful as reference Impurity standard.
    (2,4-Diaminopteridine-6,7-diyl)dimethanol
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    Product Name : ethyl 4-(methylamino)benzoate ;4-Ethoxycarbonyl-N-methylaniline; Ethyl 4-(Methylamino)benzoate; Ethyl p-(Methylamino)benzoate |10541-82-9

    Code: JCMTT-20
    CAS: 10541-82-9
    Chemical Formula: C10H13NO2
    Molecular Weight: 179.22
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: ethyl 4-(methylamino)benzoate ;4-Ethoxycarbonyl-N-methylaniline; Ethyl 4-(Methylamino)benzoate; Ethyl p-(Methylamino)benzoate |10541-82-9  is useful as reference Impurity standard.
    ethyl 4-(methylamino)benzoate ;4-Ethoxycarbonyl-N-methylaniline; Ethyl 4-(Methylamino)benzoate; Ethyl p-(Methylamino)benzoate |10541-82-9
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    Product Name : Methotrexate EP Impurity L ;(2S)-2-[[4-(Methylamino)benzoyl]amino]pentanedioic acid disodium salt |52980-68-4 (diacid) ;

    Code: JCMTT-19
    CAS: 52980-68-4 (diacid)
    Chemical Formula: C13H16N2O5 (diacid) ;C13H14N2Na2O5 (disodium salt)
    Molecular Weight: 280.28 ;324.24
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity L ;(2S)-2-[[4-(Methylamino)benzoyl]amino]pentanedioic acid disodium salt |52980-68-4 (diacid) ; is useful as reference Impurity standard.
    Methotrexate EP Impurity L ;(2S)-2-[[4-(Methylamino)benzoyl]amino]pentanedioic acid disodium salt |52980-68-4 (diacid) ;
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    Product Name : Methotrexate EP Impurity K ; (2S)-2-[(4-Aminobenzoyl)amino]pentanedioic acid ; N-(4-Aminobenzoyl)-L-glutamic Acid |4271-30-1

    Code: JCMTT-18
    CAS: 4271-30-1
    Chemical Formula:C12H14N2O5
    Molecular Weight:266.25 
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate EP Impurity K ; (2S)-2-[(4-Aminobenzoyl)amino]pentanedioic acid ; N-(4-Aminobenzoyl)-L-glutamic Acid |4271-30-1is useful as reference Impurity standard.
    Methotrexate EP Impurity K ; (2S)-2-[(4-Aminobenzoyl)amino]pentanedioic acid ; N-(4-Aminobenzoyl)-L-glutamic Acid |4271-30-1
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    Product Name : Methotrexate EP Impurity J ; Dimethyl(2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]pentanedioate ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid dimethyl ester ; Dimethyl Methotrexate | 34378-65-9

    Code: JCMTT-17
    CAS:  34378-65-9
    Chemical Formula:
    Molecular Weight:
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity J ; Dimethyl(2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]pentanedioate ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid dimethyl ester ; Dimethyl Methotrexate | 34378-65-9 is useful as reference Impurity standard.
    Methotrexate EP Impurity J ; Dimethyl(2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]pentanedioate ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid dimethyl ester ; Dimethyl Methotrexate | 34378-65-9
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    Product Name : Methotrexate EP Impurity I;Methotrexate USP RC I ; Methotrexate 1-Methyl Ester ; Methotrexate α-Methyl Ester ; (4S)-4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |66147-29-3

    Code: JCMTT-16
    CAS: 66147-29-3
    Chemical Formula:C21H24N8O5
    Molecular Weight: 468.47
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity I;Methotrexate USP RC I ; Methotrexate 1-Methyl Ester ; Methotrexate α-Methyl Ester ; (4S)-4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |66147-29-3 is useful as reference Impurity standard.
    Methotrexate EP Impurity I;Methotrexate USP RC I ; Methotrexate 1-Methyl Ester ; Methotrexate α-Methyl Ester ; (4S)-4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |66147-29-3
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    Product Name : Methotrexate EP Impurity H ;Methotrexate USP RC H ; Methotrexate 5-Methyl Ester ; Methotrexate γ-Methyl Ester ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |67022-39-3

    Code: JCMTT-15
    CAS: 67022-39-3
    Chemical Formula: C21H24N8O5
    Molecular Weight: 468.47
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity H ;Methotrexate USP RC H ; Methotrexate 5-Methyl Ester ; Methotrexate γ-Methyl Ester ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |67022-39-3 is useful as reference Impurity standard.
    Methotrexate EP Impurity H ;Methotrexate USP RC H ; Methotrexate 5-Methyl Ester ; Methotrexate γ-Methyl Ester ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]-5-methoxy-5-oxopentanoic acid |67022-39-3
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    Product Name : Methotrexate EP Impurity G ; Methotrexate USP RC G ; (2S)-2-[[4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]methylamino]benzoyl]amino]pentanedioic acid ;

    Code: JCMTT-14
    CAS: 
    Chemical Formula: C28H29N9O6
    Molecular Weight: 587.59
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate EP Impurity G ; Methotrexate USP RC G ; (2S)-2-[[4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]methylamino]benzoyl]amino]pentanedioic acid ;is useful as reference Impurity standard.
    Methotrexate EP Impurity G ; Methotrexate USP RC G ; (2S)-2-[[4-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]methylamino]benzoyl]amino]pentanedioic acid ;
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    Product Name : Methotrexate EP Impurity F ; Methotrexate USP RC F ; (R)-Methotrexate ; (2R)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid |51865-79-3

    Code: JCMTT-13
    CAS: 51865-79-3
    Chemical Formula: C20H22N8O5
    Molecular Weight: 454.44
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity F ; Methotrexate USP RC F ; (R)-Methotrexate ; (2R)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid |51865-79-3 is useful as reference Impurity standard.
    Methotrexate EP Impurity F ; Methotrexate USP RC F ; (R)-Methotrexate ; (2R)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid |51865-79-3
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    Product Name : Methotrexate EP Impurity E (HCl Salt) : 1 Methotrexate EP Impurity E ; Methotrexate USP RC E (HCl Salt) ; 2,4-Diamino-N10-methylpterioc acid hemihydrochloride salt ; 4-[[(2,4-Diaminopteridin-6-yl)methyl]methyl amino]benzoic acid hemihydrochloride salt |70844-48-3 (hemihydrochloride salt) ;

    Code: JCMTT-12
    CAS: 70844-48-3 (hemihydrochloride salt) ;
    Chemical Formula: C15H15N7O2.1/2HCl
    Molecular Weight: 343.56
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate EP Impurity E (HCl Salt) : 1 Methotrexate EP Impurity E ; Methotrexate USP RC E (HCl Salt) ; 2,4-Diamino-N10-methylpterioc acid hemihydrochloride salt ; 4-[[(2,4-Diaminopteridin-6-yl)methyl]methyl amino]benzoic acid hemihydrochloride salt |70844-48-3 (hemihydrochloride salt) ;is useful as reference Impurity standard.
    Methotrexate EP Impurity E (HCl Salt) : 1 Methotrexate EP Impurity E ; Methotrexate USP RC E (HCl Salt) ; 2,4-Diamino-N10-methylpterioc acid hemihydrochloride salt ; 4-[[(2,4-Diaminopteridin-6-yl)methyl]methyl amino]benzoic acid hemihydrochloride salt |70844-48-3 (hemihydrochloride salt) ;
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    Product Name : Methotrexate EP Impurity E (Base) ; Methotrexate USP RC E ; 2,4-Diamino-N10-methylpterioc acid ; 4-[[(2,4-Diaminopteridin-6-yl) methyl] methyl amino]benzoic acid ;

    Code: JCMTT-11
    CAS: 19741-14-1
    Chemical Formula: C15H15N7O2
    Molecular Weight: 325.33
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity E  (Base); Methotrexate USP RC E ; 2,4-Diamino-N10-methylpterioc acid ; 4-[[(2,4-Diaminopteridin-6-yl) methyl] methyl amino]benzoic acid |19741-14-1 is useful as reference Impurity standard.
    Methotrexate EP Impurity E (Base) ; Methotrexate USP RC E ; 2,4-Diamino-N10-methylpterioc acid ; 4-[[(2,4-Diaminopteridin-6-yl) methyl] methyl amino]benzoic acid ;
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    Product Name : Methotrexate EP Impurity D Desmethyl Impurity ;Folic Acid EP Impurity D ; Pteroic Acid ; 4-[[(2-Amino-4-hydroxypteridin-6-yl)methyl]amino]benzoic acid | 119-24-4

    Code: JCMTT-10
    CAS:  119-24-4
    Chemical Formula:C14H12N6O3
    Molecular Weight:312.28
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity D Desmethyl Impurity ;Folic Acid EP Impurity D ; Pteroic Acid ; 4-[[(2-Amino-4-hydroxypteridin-6-yl)methyl]amino]benzoic acid | 119-24-4 is useful as reference Impurity standard.
    Methotrexate EP Impurity D Desmethyl Impurity ;Folic Acid EP Impurity D ; Pteroic Acid ; 4-[[(2-Amino-4-hydroxypteridin-6-yl)methyl]amino]benzoic acid | 119-24-4
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    Product Name : Methotrexate EP Impurity D ; N10-Methylpteroic acid ; 4-[[(2-amino-4-hydroxypteridin-6-yl)methyl] methylamino]benzoic acid |5623-18-7

    Code: JCMTT-09
    CAS: 5623-18-7
    Chemical Formula:C15H14N6O3
    Molecular Weight: 326.31
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate EP Impurity D ; N10-Methylpteroic acid ; 4-[[(2-amino-4-hydroxypteridin-6-yl)methyl] methylamino]benzoic acid |5623-18-7is useful as reference Impurity standard.
    Methotrexate EP Impurity D ; N10-Methylpteroic acid ; 4-[[(2-amino-4-hydroxypteridin-6-yl)methyl] methylamino]benzoic acid |5623-18-7
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    Product Name : Methotrexate EP Impurity C ; Methotrexate USP RC C ; Methopteridine ; Methotrextae 4-Oxo Analog ; �N-Methylfolic acid ; (2S)-2-[[4-[[(2-amino-4-hydroxy pteridin-6-yl) methyl] methyl amino] benzoyl] amino] pentanedioic acid ;

    Code: JCMTT-08
    CAS: 2410-93-7
    Chemical Formula:C20H21N7O6
    Molecular Weight: 455.42
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity C ; Methotrexate USP RC C ; Methopteridine ; Methotrextae 4-Oxo Analog ; �N-Methylfolic acid ; (2S)-2-[[4-[[(2-amino-4-hydroxy pteridin-6-yl) methyl] methyl amino] benzoyl] amino] pentanedioic acid |2410-93-7 is useful as reference Impurity standard.
    Methotrexate EP Impurity C ; Methotrexate USP RC C ; Methopteridine ; Methotrextae 4-Oxo Analog ; �N-Methylfolic acid ; (2S)-2-[[4-[[(2-amino-4-hydroxy pteridin-6-yl) methyl] methyl amino] benzoyl] amino] pentanedioic acid ;
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    Product Name : Methotrexate EP Impurity B ; Methotrexate USP Related Compound B ; N-Desmethyl Methotrexate ; Aminopteridine ; Aminopterin ; 4-Aminofolic acid ; 4-Aminopteroyl-L-glutamic acid ; (2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid ;

    Code: JCMTT-07
    CAS:  54-62-6
    Chemical Formula: C19H20N8O5
    Molecular Weight: 440.41
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate EP Impurity B ; Methotrexate USP Related Compound B ; N-Desmethyl Methotrexate ; Aminopteridine ; Aminopterin ; 4-Aminofolic acid ; 4-Aminopteroyl-L-glutamic acid ; (2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid | 54-62-6; is useful as reference Impurity standard.
    Methotrexate EP Impurity B ; Methotrexate USP Related Compound B ; N-Desmethyl Methotrexate ; Aminopteridine ; Aminopterin ; 4-Aminofolic acid ; 4-Aminopteroyl-L-glutamic acid ; (2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid ;
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    Product Name : Methotrexate EP Impurity A ; 2,4-Diamino-6-pteridinemethanol ; (2,4-diaminopteridin-6-yl)methanol ; 2,4-Diamino-6-(hydroxymethyl) pteridine ;945-24-4

    Code: JCMTT-06
    CAS: 945-24-4
    Chemical Formula: C7H8N6O
    Molecular Weight: 192.18
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate EP Impurity A ; 2,4-Diamino-6-pteridinemethanol ; (2,4-diaminopteridin-6-yl)methanol ; 2,4-Diamino-6-(hydroxymethyl) pteridine ;945-24-4is useful as reference Impurity standard.
    Methotrexate EP Impurity A ; 2,4-Diamino-6-pteridinemethanol ; (2,4-diaminopteridin-6-yl)methanol ; 2,4-Diamino-6-(hydroxymethyl) pteridine ;945-24-4
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    Product Name : Methotrexate ;(+)-Amethopterin ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methyl amino] benzoyl]-L-glutamic acid ; 4-Amino-N10-methylpteroyl glutamic acid ; 4-Amino-N10-methylfolic acid |59-05-2

    Code: JCMTT-05
    CAS: 59-05-2
    Chemical Formula:C20H22N8O5
    Molecular Weight: 454.44
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate ;(+)-Amethopterin ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methyl amino] benzoyl]-L-glutamic acid ; 4-Amino-N10-methylpteroyl glutamic acid ; 4-Amino-N10-methylfolic acid |59-05-2 is useful as reference Impurity standard.
    Methotrexate ;(+)-Amethopterin ; (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methyl amino] benzoyl]-L-glutamic acid ; 4-Amino-N10-methylpteroyl glutamic acid ; 4-Amino-N10-methylfolic acid |59-05-2
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    Product Name : Methotrexate Dimethylamide ;(S)-2-(4-(((2,4-Diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-(dimethylamino)-5-oxopentanoic acid ;71074-44-7 ;

    Code: JCMTT-04
    CAS: 71074-44-7
    Chemical Formula:C22H27N9O4
    Molecular Weight: 481.51
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate Dimethylamide ;(S)-2-(4-(((2,4-Diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-(dimethylamino)-5-oxopentanoic acid ;71074-44-7 ; is useful as reference Impurity standard.
    Methotrexate Dimethylamide ;(S)-2-(4-(((2,4-Diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-(dimethylamino)-5-oxopentanoic acid ;71074-44-7 ;
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    Product Name : Methotrexate 7-Hydroxy Metabolite; 7-Hydroxy Methotrexate ;7-Hydroxy Amethopterin ;4-Amino-7-hydroxy-10-methylpteroylglutamic acid ;5939-37-7 ;

    Code: JCMTT-03
    CAS: 5939-37-7
    Chemical Formula:C20H22N8O6
    Molecular Weight:470.44
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate 7-Hydroxy Metabolite; 7-Hydroxy Methotrexate ;7-Hydroxy Amethopterin ;4-Amino-7-hydroxy-10-methylpteroylglutamic acid ;5939-37-7 ;
    Methotrexate 7-Hydroxy Metabolite; 7-Hydroxy Methotrexate ;7-Hydroxy Amethopterin ;4-Amino-7-hydroxy-10-methylpteroylglutamic acid ;5939-37-7 ;
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    Product Name : Methotrexate 7-Cyano Dimethyl Ester ;(S)-Dimethyl 2-(4-(((2,4-diamino-7-cyanopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate ; 112163-39-0

    Code: JCMTT-02
    CAS:  112163-39-0
    Chemical Formula:C23H25N9O5
    Molecular Weight: 507.5
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application:Methotrexate 7-Cyano Dimethyl Ester ;(S)-Dimethyl 2-(4-(((2,4-diamino-7-cyanopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate ; 112163-39-0 is useful as reference Impurity standard.
    Methotrexate 7-Cyano Dimethyl Ester ;(S)-Dimethyl 2-(4-(((2,4-diamino-7-cyanopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate ; 112163-39-0
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    Product Name : Methotrexate α-tert-Butyl Ester ;(S)-5-tert-Butoxy-4-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-oxopentanoic acid ; 79640-70-3

    Code: JCMTT-01
    CAS: 79640-70-3
    Chemical Formula:C24H30N8O5
    Molecular Weight: 510.55
    Category: Reference Standards/API Drug Metabolites/EP Impurities/USP Impurities
    Literature:
    Application: Methotrexate α-tert-Butyl Ester ;(S)-5-tert-Butoxy-4-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-oxopentanoic acid ; 79640-70-3is useful as reference Impurity standard.
    Methotrexate α-tert-Butyl Ester ;(S)-5-tert-Butoxy-4-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)-5-oxopentanoic acid ; 79640-70-3
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